CORE
COURSE XI
MOLECULAR
BIOLOGY
CODE:
ZOO-HC-5016
THEORY
(CREDITS 4)
Unit 1:Nucleic Acids
Salient features of DNA and RNA
Watson and Crick model of DNA
Unit 2:DNAReplication
DNA Replication in prokaryotes and eukaryotes,
mechanism of DNA replication,
Semi-conservative, bidirectional and semi[1]discontinuous
replication,
RNA priming,
Replication of circular and linear ds-DNA,
replication of telomeres
Unit3:Transcription
RNA polymerase and transcription Unit,
mechanism of
transcription in prokaryotes and eukaryotes,
synthesis of rRNA and mRNA,
transcription factors
Unit4:Translation
Genetic code,
Degeneracy of the genetic code and Wobble Hypothesis;
Process of protein synthesis in prokaryotes:
Ribosome structure and assembly in prokaryotes,
fidelity of protein synthesis,
aminoacyl tRNA synthetases and charging of tRNA;
Proteins involved in initiation, elongation and
termination of polypeptidechain;
Inhibitors of protein synthesis;
Difference between prokaryotic and eukaryotic
translation
Unit 5: Post Transcriptional Modifications and
Processing of Eukaryotic RNA
Structure of globin mRNA;
Split genes: concept of introns and exons,
splicing mechanism, alternative splicing, exon
shuffling, and RNA editing,
Processing of tRNA
Unit 6:GeneRegulation
Transcription regulation in prokaryotes: Principles
of transcriptional regulation with examples from lac operon and trpoperon;
Transcription Pyrimidine dimerization and mismatch repair
Unit 8: Regulatory RNAs
Ribo -switches,
RNA interference, miRNA, siRNA
